Question4:-Give the right sequence of RT-PCR

(i) Taq polymerase extends target sequences

(11) Extract poly(A)+RNA

(iii) Oligo primers bind to target sequences

(iv) Extend with reverse transcriptase to form cDNA
(v) ds cDNA denaturated by heating to >94°C

)۷ Anneal Poly(dT) primer

A:-(ii), (vi), (iv), (v), (111), (i)
B:-(v), (iii), (i), (ii), (vi), (iv)
C:-(ii), (vi), (v), (iii), (i), (iv)
D:-(i), (iii), (v), (ii), (iv), (wi)
Correct Answer:- Option-A

Question5:-Which of the following is true for achromat objective lenses in a
microscope?

(i) They are cheapest objective lens

(ii) They are corrected for spherical aberrations with green light

(111) They are best used when the light source utilizes a green filter and images are
collected in grayscale

(iv) They are spherically corrected for three wavelengths

A:-(i), (ii), (iv)
B:-(i), (iii), (iv)
C:-(ii), (iii), (iv)
D:-(i), (ii), (iii)
Correct Answer:- Option-D
Question6:-Select the correct pair of luminescence system and measured moiety.

A:-Firefly luminescence-ATP, bacterial luminescence -#,0., Chemiluminescence-
NADH

B:-Firefly luminescence- #,0,,bacterial luminescence-NADH,
Chemiluminescence-ATP

C:-Firefly luminescence-ATP, bacterial luminescence-NADH,
Chemiluminescence-#.0,

D:-Firefly luminescence-NADPH, bacterial luminescence-ATP,
Chemiluminescence-#.0,

Correct Answer:- Option-C
Question7:-Choose the right facts in Connection to PAGE

A:-Stacking gel has 5-10% acylamide and pH 8.3, Running gel has 2-3%
acrylamide and pH 6.7

B:-Stacking gel has 2-3% acylamide and pH 6.7, Running gel has 5-10%
acrylamide and pH 8.3

C:-Stacking gel has 5-10% acylamide and pH 6.7, Running gel has 2-3%
acrylamide and pH 8.3

D:-Stacking gel has 2-3% acylamide and pH 8.3, Running gel has 5-10%